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How a MICROLAB 500 diluter works
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Simple dilutions can be made in three easy steps (after priming the instrument) as shown in Figure 1:
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Figure 1.
Figure 1: A simple dilution
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Step 1: Fill the left syringe with the programmed amount of solvent (diluent) from the reservoir.

Step 2: Aspirate the programmed amount of concentrated sample into the end of the probe using the right syringe.

Step 3: Dispense the sample and solvent into a vial to complete the dilution.
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spacerIn addition to simple dilutions performed on all MICROLAB 500 diluters, models ML530B, ML531C and ML560C instruments can also perform serial, and multi-sample/reagent (or internal standard addition) dilutions. See Figure 2.
ML500 Series Method Illustration
Method type A B C
1. Simple dilution
2. Pipette
(w/disposable tip)		 dil1
Serial dilution
(programmed)		 dil2
Serial dilution
(tube to tube)		 dil3
Multi-sample/
reagent dilution
(or internal standard addition)		 dil4
Return to reservoir dil5
Figure 2:
Dilution method types		 • Blue = diluent
• Yellow = sample
• Orange = standard
• Green = diluted sample
• Purple = diluted sample + standard